Biology

DNA: Components and Amount of DNA in different species with Importance

How DNA acts as Hereditary Material?

DNA and RNA are two types of nucleic acid.The polymers of nucleotides are called nucleic acids. The unit of nucleic acid is nucleotide. Nucleic acids are the special compounds, produced in the nucleus of cell. These are composed of Carbon, hydrogen, Oxygen, Nitrogen and Phosphorus.

Discovery of Nucleic Acids

F. Miescher isolated the nucleic acid from the nucleus of pus cells in 1870. As they were isolated from nucleus and they were acidic in nature, so they were named as nucleic acid.

Types of nucleic acid

There are two types of nucleic acids:

DNA (deoxyribonucleic acid)

DNA is present in chromosomes, in the nucleus of cells. A small amount of DNA is also present in mitochondria and chloroplast.

RNA (ribonucleic acid)

RNA is present in the nucleolus, ribosome and cytosol of cytoplasm. Small amount of RNA is also present in other part of the cell.

Structure of Nucleic Acid

Nucleic acids are complex substances. They are polymers of units called nucleotides. DNA is composed of deoxyribonucleotides and RNA is composed of ribonucleotides.structure of nucleic acid

Nucleotides (nitrogen base + pentose sugar + phosphoric acid)

Each nucleotide is made up of three subunits pentose sugar, nitrogen base and phosphoric acid.

Pentose Sugar

It is five carbon monosaccharide. The nucleotides of RNA contain ribose sugar. On the other hand, the nucleotides of DNA contain Deoxyribose sugar.

Nitrogen Base

Nitrogen bases are of two types.

  • Pyrimidines: These are composed of single ring. These are Cytosine (C), Thymine (T) and Uracil (U).
  • Purines: These are composed of double rings. These are Adenine (A) and Guanine (G).

Phosphoric Acid

It has the ability to form ester linkage with OH group of Pentose Sugar. The nitrogen base is attached to the carbon 1 of the pentose sugar in a typical nucleotide. The phosphoric acid is attached with the carbon 3 of the pentose sugar. ATP (adenosine triphosphate) is also an important nucleotide. The cells use ATP as energy currency.

Nucleoside (Nitrogen Base + Pentose Sugar)

The compound formed by the nitrogenous base and pentose sugar is called nucleoside. A nucleoside and phosphoric acid combine to form a nucleotide.nucleoside

DNA (deoxyribonucleic acid)

DNA is the hereditary material. It controls the properties and activities of a cell.

Components of DNA

DNA is made of four kinds of Nucleotides. These are:

  1. d-adenosine monophosphate (d-AMP).
  2. d-guanosine monophosphate (d-GMP).
  3. d-cytidine monophosphate (d-CMP).
  4. d-thymidine monophosphate (d-TMP).

The nucleotides are linked with one another by phosphodiester bonds and form a long chain. This chain is called Polynucleotide chain. This chain has a specific sequence. There may be different lengths of nucleotide chain.

·         Dinucleotide

Two nucleotides join to form dinucleotide. Nicotinamide adenine dinucleotide (NAD) is a dinucleotide. It is important coenzyme. It takes part in several oxidation-reduction reactions in the cell.

·         Trinucleotides

Three nucleotides join to form Trinucleotides.

·         Polynucleotides

Many nucleotides join to form polynucleotide.

Ratio of bases in DNA

Erwin Chargaff provided a data about the ration of different bases of DNA molecule. It shows that adenine and Thymine have equal ratio and guanine and cytosine have equal ration.ratio of bases in DNA

Watson and Crick model of DNA

Maurice Wilkins and Rosalind Franklin determine the structure of DNA by X-ray diffraction technique. Finally, James D. Watson and Francis Crick proposed the model of DNA. It suggests:

  • The DNA is made up of two polynucleotide chains or strands.
  • These two strands coil around each other and from a double helix.
  • The coiling of the two strands is opposite (antiparallel to each other i.e., one strand is 3-5 the other is 5-3).
  • The two strands are attached with each other by weak hydrogen bonds. Two hydrogen bonds are formed between A and T and three hydrogen bonds are formed between G and C pairs.
  • Adenine (A) is always opposite to Thymine (T) and Guanine (G) is always opposite to Cytosine.
  • The two strands coil around each other. Each turn of the strands is about 34 Angstrom. This turn contains 10 base pairs.DNA structure

Amount of DNA in different Species

The amount of DNA depends on the number of chromosomes in a species. Each species has fix number of chromosomes. So the amount of DNA is also fixed for a particular species. The germ cells (sperm and ova) contain one half the amounts of the somatic cells (body cells).

DNA in different cells

Importance of DNA

All the information for the structure and the functioning of a cell are stored in DNA. For example, E.coli (bacteria) contains single chromosome. This chromosome contains 5 million bases. These bases are arranged in a particular linear order. These bases contain information. This information is present in the forms of units called “gene”. A gene is a small part of DNA that controls a specific character. Gene is unit of biological inheritance.

The E.coli genome (total number of genes) consists of 4, 639, 221 base pairs. This genome codes for 4288 proteins.

Haemophilus influenza (a bacteria that causes influenza is the first microbe that genome have been completely sequenced. This sequencing was published in 1995).

DNA as Hereditary Material:

DNA is found in gene and chromosomes. It is considered as genetic material because it transfers the hereditary character into new cell or new generation. The property of DNA as hereditary material can be proved by two experiments:

Griffith Experiment:

Griffith proved DNA as genetic material by transformation process in bacteria. In this process the living bacteria can get genetic material (DNA) from dead bacteria and can be transformed (changed) from harmless to harmful virulent bacteria. It shows that DNA acts as genetic material.

Hershey and Chase Experiment:


Hershey and Chase presented another proof of DNA as genetic material. According to their experiment it is proved that the genetic material (DNA) of one bacterial cell can be transferred into another bacterial cell by bacterio phage virus.

They explained that when bacterio phage virus attacks bacterial cell only its DNA enters the bacterial cell and its outer protein coat remains outside. The DNA of virus takes the biochemical control of bacterial cell and starts to prepare its DNA. After that, the complete body of virus is formed. It indicates that DNA acts as genetic material.

Function of DNA

The four nitrogen bases of DNA take part in genetic code. These are arranged in straight manner along the DNA strand. In plants and animals numerous information can be encoded in the form of genetic code.

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